Composite

Part:BBa_K5226074:Design

Designed by: Yujiao Yang   Group: iGEM24_SCUT-China-A   (2024-09-05)


B0064-porin281-Vib-ftl-porin194-Vib-folD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1189
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1189
    Illegal NheI site found at 2375
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1189
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1189
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1189
    Illegal NgoMIV site found at 455
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

PCR amplification.Sequence source is from Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759, found through NCBI （https://www.ncbi.nlm.nih.gov/nuccore/CP009977.1）

References